• Freezing and preserving blood, tissues, semen and other biological specimens.
• Cryosurgery and cryopreservation
• A source of pneumatic pressure to power gas-operated medical devices
• As a coolant for Carbon Dioxide surgical lasers.
• As a component in many gas mixtures:
A displacement medium for sterile equipment.
A non-oxidizing displacement medium in pharmaceutical vials.
A propellant in pressurised aerosol type dispensers